# FrontierScience / af36a1cb-5949-4385-93a0-1f875500c34a

task_id: f21b6218-5830-5a99-80e3-4efb7f608ebf
task_key: olympiad--test--af36a1cb~2d5949~2d4385~2d93a0~2d1f875500c34a
task_revision_id: 1

{"problem":"An experimental design involves coating a glass slide with a supported lipid bilayer (SLB). After the slide is prepared, 20 nt-long 5'-cholesterol-modified ssDNA strands are added to the slide and incubation at room temperature is performed before the slide is repeatedly washed with a buffer that contains Tris, EDTA, and Tween 0.05%. Afterwards, a solution containing ATTO532-modified ssDNA strands with 9 nt complementarity (22% GC content) to the previously mentioned strands are added and the slides are imaged using an EMCCD camera linked to a microscope with the appropriate laser line and total internal reflection (TIR) illumination at room temperature. When imaged with 100 ms exposure time, there are clearly distinct blinking spots that correspond to binding events, but when imaged with 1 ms exposure, there is no discernible signal at all. Given this design, what is the main source of noise in the 1 ms image?\n\nThink step by step and solve the problem below. At the end of your response, write your final answer on a new line starting with “FINAL ANSWER”. It should be an answer to the question such as providing a number, mathematical expression, formula, or entity name, without any extra commentary or providing multiple answer attempts.","subject":"biology"}

Source: https://huggingface.co/datasets/openai/frontierscience

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