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FrontierScience / 111d5872-2705-42c0-8df4-0fb386ba7084 / HEK293T cells were treated with 2.5 µM CPT, a DNA topoisomerase I inhibitor,…
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problem
HEK293T cells were treated with 2.5 µM CPT, a DNA topoisomerase I inhibitor, after which the RNA for gene X was quantified with RT-PCR and agarose gel electrophoresis. The results of this reaction show a band 2 kb in size, even though computational analysis of the genomic sequence of gene X suggests a 2300 bp product. Next, the experiment was repeated, but this time the cells were also treated with 1 µM trichostatin A (TSA). This time, the resulting electrophoresis result shows a strong band at 2.3 kb, along with a faint band at 2000 bp. Based on these experiments, what process appears to be the main driver of gene X alternative splicing? Think step by step and solve the problem below. At the end of your response, write your final answer on a new line starting with “FINAL ANSWER”. It should be an answer to the question such as providing a number, mathematical expression, formula, or entity name, without any extra commentary or providing multiple answer attempts.
subject
biology
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