FrontierScience / 1510d6bd-6785-456d-8988-b9382a3aef9c / An 8 kbp plasmid was extracted from an unknown bacterium using a silica…

Problem

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An 8 kbp plasmid was extracted from an unknown bacterium using a silica membrane-based approach. For the first characterization of the plasmid, the researcher mixed the extracted plasmid with glycerol and an intercalating dye and proceeded to run electrophoresis on a 1% agarose gel. The results of the electrophoresis resulted in five distinct bands: one at the 8 kbp mark; one slightly higher; one very high but faint band; and another two separate bands significantly lower, with the higher of these two being the brightest of the five total bands. Using Southern blots and a known 40-mer sequence, the researcher concluded that all five bands came from the plasmid. The researcher also performed absorbance experiments and found that the lowest band absorbed almost twice as much 260 nm light as the other bands. Based on this information, please name the form of the DNA molecules from the highest to lowest band.

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biology

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Source and history

upstream_id / Original problem ID

1510d6bd-6785-456d-8988-b9382a3aef9c

task_id

d8880921-2601-55b3-b804-4860dbe1a948

task_key

olympiad--test--1510d6bd~2d6785~2d456d~2d8988~2db9382a3aef9c

task_revision_id

1

config / split

olympiad / test

Official source

initial import