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FrontierScience / af36a1cb-5949-4385-93a0-1f875500c34a / An experimental design involves coating a glass slide with a supported lipid…

Problem

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problem

An experimental design involves coating a glass slide with a supported lipid bilayer (SLB). After the slide is prepared, 20 nt-long 5'-cholesterol-modified ssDNA strands are added to the slide and incubation at room temperature is performed before the slide is repeatedly washed with a buffer that contains Tris, EDTA, and Tween 0.05%. Afterwards, a solution containing ATTO532-modified ssDNA strands with 9 nt complementarity (22% GC content) to the previously mentioned strands are added and the slides are imaged using an EMCCD camera linked to a microscope with the appropriate laser line and total internal reflection (TIR) illumination at room temperature. When imaged with 100 ms exposure time, there are clearly distinct blinking spots that correspond to binding events, but when imaged with 1 ms exposure, there is no discernible signal at all. Given this design, what is the main source of noise in the 1 ms image?

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subject

biology

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Source and history

upstream_id / Original problem ID: af36a1cb-5949-4385-93a0-1f875500c34a
task_id: f21b6218-5830-5a99-80e3-4efb7f608ebf
task_key: olympiad--test--af36a1cb~2d5949~2d4385~2d93a0~2d1f875500c34a
task_revision_id: 1
config / split: olympiad / test

Official source

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