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MMLU-Pro / 3359 / A strain of Salmonellatyphimuriumhas a base pair substitutionwhich makes it…

Problem

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question

A strain of Salmonellatyphimuriumhas a base pair substitutionwhich makes it His^-. This strain's rate of spontaneousreversion to His^+ is 5 × 10^-8. Chemical X is addedto the growth medium of a culture of these cells. Solutions are diluted and plated onto minimal medium (a10^-2 dilution) and onto nutrient rich medium (a10^-3 dilution). The plates are incubated for 48 hours. The coloniesare counted: 50 colonies on the minimal medium and1,000 colonies on the nutrient rich medium. Whatcan youconclude from this experiment?

category

biology

options

  1. Chemical X has no effect on mutation rate
  2. Chemical X repairs the base pair substitution in the bacteria
  3. Chemical X only allows growth of His^- cells on minimal medium
  4. Chemical X inhibits growth on minimal medium
  5. Chemical X is beneficial for the bacteria
  6. Chemical X promotes growth on nutrient rich medium only
  7. Chemical X selectively kills non-mutant bacteria
  8. Chemical X increases mutation rate for other genes but not His
  9. Chemical X decreases the mutation rate
  10. Chemical X is highly mutagenic

src

stemez-Genetics

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